In the interest of reproducibility and transparency, we endeavor to make our publications “standalone”:
Raw NGS data is uploaded to ENA
Some intermediate NGS analyses are given DOIs through Zenodo or e!DAL
Plasmids are deposited in Addgene
Germplasm is made available from public good repositories, e.g. the Germplasm Resources Unit or Wheat Genetics Resources Center
Computer script is made available through GitHub
We have also published detailed protocol papers for many of the methods we have implemented.
Germplasm
Code
Methods
Mago et al. (2017). Generation of loss-of-function mutants for wheat rust disease resistance gene cloning. In “Wheat Rust Diseases: Methods and Protocols”, Springer Molecular Biology Protocols Database. Edited by Sambasivam Periyannan. Pages 199-206.
Yu et al. (2017). Isolation of wheat genomic DNA for gene mapping and cloning. In “Wheat Rust Diseases: Methods and Protocols”, Springer Molecular Biology Protocols Database. Edited by Sambasivam K Periyannan. Pages 207-214.
Steuernagel et al. (2017). MutRenSeq – a method for rapid cloning of plant disease resistance genes. In “Wheat Rust Diseases: Methods and Protocols”, Springer Molecular Biology Protocols Database. Edited by Sambasivam K Periyannan. Pages 215-230.
Steuernagel et al. (2017). Rapid gene isolation using MutChromSeq. In “Wheat Rust Diseases: Methods and Protocols”, Springer Molecular Biology Protocols Database. Edited by Sambasivam K Periyannan. Pages 231-244.
Arora et al. (2019). Resistance gene cloning from a wild crop relative by sequence capture and association genetics. Nature Biotechnology 37:139–143.
Gaurav et al. (2022). Population genomic analysis of Aegilops tauschii identifies targets for bread wheat improvement. Nature Biotechnology 40:422-431.
EMS mutagenesis of wheat stem rust spores